Kiho Lee

PhD - Reproductive Physiology, 2010, Purdue University
MS - Reproductive Physiology, 2005, Purdue University
BS - Animal Science and Technology, 2003, Seoul National University

Dr. Lee’s Lab currently focuses on early embryonic development in pigs. Understanding mechanisms underlying the dynamic changes that occur during embryonic development can lead us to develop more efficient ways to generate pig embryos thus increasing overall productivity. Pigs have great value in agriculture and also in biomedicine as pigs share a similar physiology to humans. Major topics in the laboratory are:

1. Identifying the mechanism of epigenetic reprogramming by oocytes after fertilization;

2. Developing an efficient approach to produce genetically engineered pig models for agriculture and biomedical research;

3. Developing new methods of generating in vitro-derived embryos with higher developmental competency.

Lee K, Hamm J, Whitworth K, Spate L, Park KW, Murphy CN, Prather RS. Dynamics of TET family expression in porcine preimplantation embryos is related to zygotic genome activation and required for the maintenance of NANOG. Dev Biol.  2014 Feb 1;386(1):86-95.

Prather RS, Rowland RR, Ewen C, Trible B, Kerrigan M, Bawa B, Teson JM, Mao J, Lee K, Samuel MS, Whitworth KM, Murphy CN, Egen T, Green JA. An intact sialoadhesin (Sn/SIGLEC1/CD169) is not required for attachment/internalization of the porcine reproductive and respiratory syndrome virus. J Virol. 2013 Sep;87(17):9538-46.  

Kwon DN, Lee K, Kang MJ, Choi YJ, Park CK, Whyte JJ, Brown AN, Kim JH, Samuel M, Mao J, Park KW, Murphy CN, Prather RS, and Kim JH. Production of biallelic CMP-N-acetylneuraminic acid hydroxylase knock-out pigs. Sci Rep. 2013; 3:1981.  

Lee K, Redel BK, Spate L, Teson J, Brown AN, Park KW, Walters E, Samuel M, Murphy CN, Prather RS. Piglets produced from cloned blastocysts cultured in vitro with GM-CSF. Mol Reprod Dev. 2013 Feb;80(2):145-54.

Yang X, Mao J, Walters E, Zhao MT, Teson JM, Lee K, Prather RS. Xenopus Egg Extract Treatment Reduced Global DNA Methylation of Donor Cells and Enhanced Somatic Cell Nuclear Transfer Embryo Development in Pigs. Biores Open Access. 2012 Apr;1(2):79-87.

Zhao MT, Yang X, Lee K, Mao J, Teson JM, Whitworth KM, Samuel MS, Spate LD, Murphy CN, Prather RS. The In Vivo Developmental Potential of Porcine Skin-Derived Progenitors and Neural Stem Cells. Stem Cells Dev. 2012 Sep 20;21(14):2682-8.

Lee K, Wang C, Machaty Z. STIM1 is required for Ca2+ signaling during mammalian fertilization. Dev Biol. 2012 Jul 15;367(2):154-62.

Wang C, Lee K, Gajdo´csi E, Bali Papp A, Machaty Z. Orai1 mediates store-operated Ca2+ entry during fertilization in mammalian oocytes. Dev Biol. 2012 May 15;365(2):414-23.

Lee K, Wang C, Chaille JM, Machaty Z. Effect of resveratrol on porcine embryo development. J Reprod Dev. 2010; 56(3): 330-5